ARG66033

anti-Cardiotrophin 1 antibody

anti-Cardiotrophin 1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Neutralizing,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Cardiotrophin 1
Tested Reactivity Hu
Tested Application ELISA, IHC-P, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cardiotrophin 1
Antigen Species Human
Immunogen E. coli derived recombinant Human Cardiotrophin 1.
(SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA)
Conjugation Un-conjugated
Alternate Names CT-1; CT1; Cardiotrophin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG66034 as a detection antibody
IHC-P0.25 µg/ml
Neut0.25 - 0.40 µg/ml (To yield [ND50] of the biological activity of hCardiotrophin-1 (15 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1489 Human CTF1

Swiss-port # Q16619 Human Cardiotrophin-1

Gene Symbol CTF1
Gene Full Name cardiotrophin 1
Background The protein encoded by this gene is a secreted cytokine that induces cardiac myocyte hypertrophy in vitro. It has been shown to bind and activate the ILST/gp130 receoptor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]
Function Induces cardiac myocyte hypertrophy in vitro. Binds to and activates the ILST/gp130 receptor. [UniProt]
Calculated MW 21 kDa