ARG23707

anti-Caspase 1 antibody

anti-Caspase 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Caspase 1
Tested Reactivity Hu
Tested Application IHC-P, WB
Specificity The antibody might react full length and p10 cleaved from of caspase 1 protein based on immunogen sequence design.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caspase 1
Antigen Species Human
Immunogen Synthetic peptide around aa. 348-361 within the p10 subunit of Human Caspase-1. (VFIGRLIEHMQEYA)
Conjugation Un-conjugated
Alternate Names Caspase-1; Interleukin-1 beta-converting enzyme; IL-1 beta-converting enzyme; CASP-1; ICE; IL-1BC; Interleukin-1 beta convertase; P45; IL1BC; p45; EC 3.4.22.36

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 5 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.025% Sodium azide and 2.5% BSA.
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 834 Human CASP1

Swiss-port # P29466 Human Caspase-1

Gene Symbol CASP1
Gene Full Name caspase 1, apoptosis-related cysteine peptidase
Background Caspase 1 is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms. [provided by RefSeq, Mar 2012]
Function Caspase 1: Thiol protease that cleaves IL-1 beta between an Asp and an Ala, releasing the mature cytokine which is involved in a variety of inflammatory processes. Important for defense against pathogens. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Can also promote apoptosis. Upon inflammasome activation, during DNA virus infection but not RNA virus challenge, controls antiviral immunity through the cleavage of CGAS, rendering it inactive (PubMed:28314590). In apoptotic cells, cleaves SPHK2 which is released from cells and remains enzymatically active extracellularly (PubMed:20197547). [UniProt]
Highlight Related products:
Caspase 1 antibodies; Caspase 1 ELISA Kits; Caspase 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Related news:
Exploring Antiviral Immune Response
RIP1 activation and pathogenesis of NASH
Research Area Pyroptosis Study antibody; NLRP3 Inflammasome Study antibody; NLRC4 Inflammasome Study antibody
Calculated MW 45 kDa
PTM The two subunits are derived from the precursor sequence by an autocatalytic mechanism. [UniProt]