ARG54400
anti-Caspase 10 antibody
anti-Caspase 10 antibody for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Caspase 10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | This antibody recognizes full-length human caspase-10 (59kDa). Since the sequences at C-termini of FLICE2 and Mch4 are different, this antibody recognizes only the FLICE2 form of caspase-10. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caspase 10 |
| Antigen Species | Human |
| Immunogen | Peptide corresponding to aa 505-521 at C-terminus of human FLICE2 (accession no. AAD28402). |
| Conjugation | Un-conjugated |
| Alternate Names | Caspase-10; EC 3.4.22.63; ICE-like apoptotic protease 4; Apoptotic protease Mch-4; FAS-associated death domain protein interleukin-1B-converting enzyme 2; FLICE2; CASP-10; ALPS2; MCH4 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
| Positive Control | HeLa, Jurkat, A431 and K562 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunoaffinity chroma-tography |
| Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP10 |
| Gene Full Name | caspase 10, apoptosis-related cysteine peptidase |
| Background | A novel ICE/CED-3 protease was identified recently, designated FLICE2 and Mch4, and renamed as caspase-10. Caspase-10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase-10 is, therefore, involved in CD95- and TNFR1-induced apoptosis. Caspase-10 cleaves and activates caspase-3, -4, -6, -7, -8, and -9, thereby causing proteolytic cleavage of many key proteins, such as PARP. Cleavage of PARP occurs in many different systems during apoptosis and is the hallmark of programmed cell death. Caspase-10 is expressed in many tissues and cell lines. |
| Function | Involved in the activation cascade of caspases responsible for apoptosis execution. Recruited to both Fas- and TNFR-1 receptors in a FADD dependent manner. May participate in the granzyme B apoptotic pathways. Cleaves and activates caspase-3, -4, -6, -7, -8, and -9. Hydrolyzes the small- molecule substrates, Tyr-Val-Ala-Asp-|-AMC and Asp-Glu-Val-Asp-|-AMC. Isoform C is proteolytically inactive. [UniProt] |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody |
| Calculated MW | 59 kDa |
| PTM | Cleavage by granzyme B and autocatalytic activity generate the two active subunits. |
