ARG20001
anti-Caspase 3 antibody [C33]
anti-Caspase 3 antibody [C33] for Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [C33] recognizes Caspase 3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | WB |
| Specificity | The antibody recognizes both proform and the cleaved large fragment of caspase-3 in samples of human, mouse and rat origins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C33 |
| Isotype | IgG1 |
| Target Name | Caspase 3 |
| Antigen Species | Human |
| Immunogen | Recombinant human Caspase-3 |
| Conjugation | Un-conjugated |
| Alternate Names | CPP-32; Caspase-3; EC 3.4.22.56; Apopain; CASP-3; CPP32; Cysteine protease CPP32; SCA-1; SREBP cleavage activity 1; CPP32B; Protein Yama |
Application Instructions
| Application Suggestion |
|
||||
|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified Antibody |
| Buffer | PBS, 50% Glycerol, 0.5 mg/ml BSA and 0.01% Thimerosal |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 50% Glycerol, 0.5 mg/ml BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The caspase family of cysteine proteases play a key role in apoptosis. Caspase-3 is the most extensively studied apoptotic protein among caspase family members. Caspase-3 is synthesized as inactive pro-enzyme that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kD) and small (12 kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells (e.g., PARP and DFF). |
| Highlight | Related Antibody Duos and Panels: ARG30105 Apoptosis Marker Antibody Duo (Caspase3, PARP) ARG30110 Mitochondria/Caspase dependant Apoptosis Antibody Panel (Caspase3, Caspase9, Cytochrome c, PARP) (WB) Related products: Caspase 3 antibodies; Caspase 3 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Neuroscience antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| Calculated MW | 32 kDa |
| PTM | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. |
Customer's Feedback
Good
Review for anti-Caspase 3 antibody [C33]
Application：WB
Sample：HeLa untreated / treated with Staurosporine (1μM, overnight)
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Caspase 3 antibody [C33]
Application：WB
Sample：HeLa without/with Staurosporine
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Caspase 3 antibody [C33]
Application：WB
Sample：PC-3
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：4 µg/ml
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Inhibition of histone acetylation by curcumin reduces alcohol-induced fetal cardiac apoptosis.
WB / Mouse (Since arigo failed mouse sample testing, therefore, the specie used in this paper is not covered the arigo's guarantee.)
