ARG54651
anti-Caspase 9 antibody
anti-Caspase 9 antibody for ELISA,ICC/IF,Immunoprecipitation,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Caspase 9 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ELISA, ICC/IF, IP, WB |
| Specificity | Caspase-9 antibody is predicted to have no cross reactivity to other members in the caspase family. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Caspase 9 |
| Immunogen | Synthetic peptide (20 aa) within aa. 290-340 of Human Caspase-9. |
| Conjugation | Un-conjugated |
| Alternate Names | APAF-3; ICE-LAP6; PPP1R56; CASP-9; Apoptotic protease-activating factor 3; Caspase-9; ICE-like apoptotic protease 6; Apoptotic protease Mch-6; APAF3; MCH6; EC 3.4.22.62 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Positive Control | HeLa Cell Lysate | ||||||||||
| Observed Size | 45 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS and 0.02% Sodium azide |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | CASP9 |
| Gene Full Name | caspase 9, apoptosis-related cysteine peptidase |
| Background | Caspase-9 Antibody: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues. | |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody |
| Calculated MW | 46 kDa |
| PTM | Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage. |
