ARG41157
anti-Cathepsin D antibody
anti-Cathepsin D antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Dog,Human,Monkey,Mouse,Rat
Overview
Product Description | Goat Polyclonal antibody recognizes Cathepsin D |
---|---|
Tested Reactivity | Hu, Ms, Rat, Dog, Mk |
Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
Host | Goat |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | Cathepsin D |
Antigen Species | Human |
Immunogen | Purified recombinant peptide within aa. 275 to the C-terminus of Human Cathepsin D. |
Conjugation | Un-conjugated |
Alternate Names | CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D |
Application Instructions
Application Suggestion |
|
||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Positive Control | Human (Jurkat, HT1080, HUH, MDA-MB-231, ARPE19, SH-SY5Y), Dog (MDCK) and Monkey (COS-7) whole cell lysates. |
Properties
Form | Liquid |
---|---|
Purification | Affinity purification with immunogen. |
Buffer | PBS, 0.05% Sodium azide and 20% Glycerol. |
Preservative | 0.05% Sodium azide |
Stabilizer | 20% Glycerol |
Concentration | 3 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
---|---|
Gene Symbol | CTSD |
Gene Full Name | cathepsin D |
Background | This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008] |
Function | Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt] |
Cellular Localization | Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). [UniProt] |
Calculated MW | 45 kDa |
PTM | N- and O-glycosylated. Undergoes proteolytic cleavage and activation by ADAM30. As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034). [UniProt] |