ARG55566

anti-Cathepsin D antibody

anti-Cathepsin D antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Cathepsin D

Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cathepsin D
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide from Human Cathepsin D.
Conjugation Un-conjugated
Alternate Names CPSD; EC 3.4.23.5; HEL-S-130P; CLN10; Cathepsin D

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:25
IHC-P1:25
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1509 Human CTSD

Swiss-port # P07339 Human Cathepsin D

Gene Symbol CTSD
Gene Full Name cathepsin D
Background This gene encodes a lysosomal aspartyl protease composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. This proteinase, which is a member of the peptidase C1 family, has a specificity similar to but narrower than that of pepsin A. Transcription of this gene is initiated from several sites, including one which is a start site for an estrogen-regulated transcript. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease. [provided by RefSeq, Jul 2008]
Function Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. [UniProt]
Cellular Localization Lysosome. Melanosome. Secreted, extracellular space. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 45 kDa
PTM N- and O-glycosylated.
Undergoes proteolytic cleavage and activation by ADAM30.
As well as the major heavy chain which starts at Leu-169, 2 minor forms starting at Gly-170 and Gly-171 have been identified (PubMed:1426530). An additional form starting at Ala-168 has also been identified (PubMed:27333034).

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Review for anti-Cathepsin D antibody

Application:WB

Sample:HepG2 and U87

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

Physiological β-amyloid clearance by the liver and its therapeutic potential for Alzheimer’s disease

WB / Human

Yuan Cheng et al.
Acta Neuropathol.,  (2023)

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Amyloid-beta uptake by blood monocytes is reduced with ageing and Alzheimer's disease.

WB / Human

Si-Han Chen et al.
Transl Psychiatry.,  (2020)

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