ARG57976

anti-Caveolin 1 antibody

anti-Caveolin 1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Endosome Marker antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Caveolin 1
Tested Reactivity Hu, Ms
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Caveolin 1
Antigen Species Human
Immunogen Synthetic peptide derived from Human Caveolin 1.
Conjugation Un-conjugated
Alternate Names CGL3; LCCNS; PPH3; MSTP085; VIP21; BSCL3; Caveolin-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431
Observed Size ~ 22 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12389 Mouse CAV1

GeneID: 857 Human CAV1

Swiss-port # P49817 Mouse Caveolin-1

Swiss-port # Q03135 Human Caveolin-1

Gene Symbol CAV1
Gene Full Name caveolin 1, caveolae protein, 22kDa
Background The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 mitogen-activated kinase cascade. Caveolin 1 and caveolin 2 are located next to each other on chromosome 7 and express colocalizing proteins that form a stable hetero-oligomeric complex. Mutations in this gene have been associated with Berardinelli-Seip congenital lipodystrophy. Alternatively spliced transcripts encode alpha and beta isoforms of caveolin 1.[provided by RefSeq, Mar 2010]
Function May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30310 Endosome, Lysosome, Peroxisome Marker Antibody Panel (Catalase, Caveolin1, Clathrin heavy chain, LAMP1)
Related products:
Caveolin 1 antibodies; Caveolin 1 Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Endosome Marker antibody
Calculated MW 20 kDa
PTM The initiator methionine for isoform 2 is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.

Phosphorylated at Tyr-14 by ABL1 in response to oxidative stress. [UniProt]