ARG63987

anti-ChAT antibody

anti-ChAT antibody for IHC-Frozen sections,Western blot and Mouse,Rat

Neuroscience antibody

Overview

Product Description

Goat Polyclonal antibody recognizes ChAT

Tested Reactivity Ms, Rat
Tested Application IHC-Fr, WB
Specificity This antibody is NOT expected to cross-react to Human ChAT.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name ChAT
Antigen Species Mouse
Immunogen C-KERARGPTKPSNLD
Conjugation Un-conjugated
Alternate Names CMS1A; Choline acetylase; CHOACTase; CHOACTASE; EC 2.3.1.6; CMS6; Choline O-acetyltransferase; ChAT; CMS1A2

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr1:2000
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12647 Mouse CHAT

Gene Symbol Chat
Background This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]
Research Area Neuroscience antibody
Calculated MW 83 kDa