ARG52873
anti-Claudin 10 antibody
anti-Claudin 10 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Signaling Transduction antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Claudin 10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Claudin 10 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from C-terminus of human claudin-10 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | OSP-L; CPETRL3; Claudin-10; OSP-like protein |
Application Instructions
| Application Suggestion |
|
||||||
|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
||||||
| Positive Control | Brain, Skeletal Muscle, Ovarian Carcinoma |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The claudin superfamily consists of many structurally related proteins in humans. These proteins, which include claudin 1 through 18, are important structural and functional components of tight junctions in paracellular transport. Claudins are located in both epithelial and endothelial cells in all tight junction-bearing tissues. Claudins, which consist of four trans-membrane domains and two extracellular loops, make up tight junction strands. |
| Cellular Localization | cytoplasm |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 24 kDa |
Customer's Feedback
Good
Review for anti-Claudin 10 antibody
Application：WB
Sample：Mouse kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
