ARG52248

anti-Collagen I, alpha 1 telopeptide antibody

anti-Collagen I, alpha 1 telopeptide antibody for Immunohistochemistry,Western blot and Human,Mouse,Sheep

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen I, alpha 1 telopeptide
Tested Reactivity Hu, Ms, Sheep
Predict Reactivity Amph, Bird, Mamm
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen I, alpha 1 telopeptide
Antigen Species Human
Immunogen Synthetic peptide corresponding to amino acid residues specific to the collagen I, alpha 1 telopeptide conjugated to KLH
Conjugation Un-conjugated
Alternate Names OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:1,00
WB1:1,000
Application Note Specific for the ~ 140 kDa telopeptide portion of the collagen I α1 polypeptide. The antibody works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigen-retrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citRate (pH 6.0)/ 0.1 % Tween-20). Note that in paraffin sections of formaldehydefixed fibrotic mouse lung tissue, the antibody recognizes mature collagen I that has formed fibrils in the extracellular matrix.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1277 Human COL1A1

GeneID: 12842 Mouse COL1A1

Swiss-port # P02452 Human Collagen alpha-1(I) chain

Swiss-port # P11087 Mouse Collagen alpha-1(I) chain

Gene Symbol COL1A1
Gene Full Name collagen, type I, alpha 1
Background Collagen is an extracellular matrix protein that serves as a scaffold defining the shape and mechanical properties of many tissues and organs including skin, tendon, artery walls, fibrocartilage, bone and teeth. Type 1 collagen is the must abundant protein in mammals. Collagens are synthesized with N-terminal and C-terminal propeptides that are cleaved during maturation and secretion. After cleavage of the propeptides, the most N-terminal and C-terminal remaining sequences are known as telopeptides. Mutations in the collagen 1, alpha 1 gene (COL1A1) are known to cause osteogenesis imperfecta (aka brittle bone disease) (Byers 1989). Furthermore, mutations found in the fist 90 residues of the helical region of alpha 1 collagen have been implicated in the prevention or delayed removal of the procollagen N-propeptide leading to a combined osteogenesis imperfecta and Ehlers-Danlos syndrome (EDS) phenotype (Cabral et al., 2005).
Highlight Related products:
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Rabbit IgG secondary antibodies;
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Research Area Signaling Transduction antibody
Calculated MW 139 kDa
PTM Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.