ARG21967

anti-Collagen I antibody (Biotin), pre-adsorbed

anti-Collagen I antibody (Biotin), pre-adsorbed for ELISA,Electron microscopy,Flow cytometry,FLISA,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Cat,Chicken,Dog,Elephant,Guinea pig,Hamster,Pig,Rabbit

Overview

Product Description

Biotin-conjugated Goat Polyclonal antibody recognizes Collagen I

Tested Reactivity Hu, Ms, Rat, Bov, Cat, Chk, Dog, Elp, Gpig, Hm, Pig, Rb
Tested Application ELISA, EM, FACS, FLISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity The antibody reacts with conformational determinants on type I collagen. The antibody is pre-adsorbed with Collagen types II, III, IV, V and VI, so the antibody may not react with Collagen types II, III, IV, V and VI.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Collagen I
Antigen Species Human
Immunogen Human Type I Collagen
Conjugation Biotin
Alternate Names OI1; OI2; OI3; OI4; EDSC; Collagen alpha-1(I) chain; Alpha-1 type I collagen

Application Instructions

Pre Adsorbed Collagen types II, III, IV, V and VI.
Application Suggestion
Tested Application Dilution
ELISA1:1000 - 1:4000
EMAssay-dependent
FACSAssay-dependent
FLISAAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.1% Sodium azide.
Preservative 0.1% Sodium azide
Concentration 0.4 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 1277 Human COL1A1

GeneID: 12842 Mouse COL1A1

GeneID: 282187 Bovine COL1A1

Gene Symbol COL1A1
Gene Full Name collagen, type I, alpha 1
Background This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish, Feb 2008]
Function Type I collagen is a member of group I collagen (fibrillar forming collagen). [UniProt]
Highlight Related products:
Collagen I antibodies; Collagen I ELISA Kits; Collagen I Duos / Panels; Anti-Goat IgG secondary antibodies;
Related news:
New antibody panels for Myofibroblasts and CAFs
Calculated MW 139 kDa
PTM Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.
O-linked glycan consists of a Glc-Gal disaccharide bound to the oxygen atom of a post-translationally added hydroxyl group.