ARG23680
anti-Collagen II (Cyanogen Bromide Fragments) antibody
anti-Collagen II (Cyanogen Bromide Fragments) antibody for ELISA,Flow cytometry,Western blot,ICC/IF,Immunohistochemistry,Immunoprecipitation,Radioimmunoassay and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Collagen II (Cyanogen Bromide Fragments) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, IHC, IP, RIA, WB |
| Specificity | The antibody reacts with collagen type II from human, mouse and rat. It exhibits very moderately cross reactivity with bovine collagen type XI and slightly with collagen type I (< 10%). |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Collagen II (Cyanogen Bromide Fragments) |
| Antigen Species | Mouse |
| Immunogen | Collagen type II BrCN fragment. |
| Conjugation | Un-conjugated |
| Alternate Names | AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen) |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | The IgG fraction was purified from rabbit neat serum by affinity chromatography. |
| Buffer | PBS |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | COL2A1 |
| Gene Full Name | collagen, type II, alpha 1 |
| Background | This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008] |
| Function | Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt] |
| Calculated MW | 142 kDa |
| PTM | Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains. The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. [UniProt] |
