ARG40202

anti-Collagen II antibody

anti-Collagen II antibody for IHC-Frozen sections and Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen II
Tested Reactivity Rat
Predict Reactivity Ms, Hm
Tested Application IHC-Fr
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen II
Antigen Species Mouse
Immunogen Synthetic peptide corresponding to aa. 1222-1241 of Mouse Collagen II. (AFAGLGQREKGPDPMQYMRA)
Conjugation Un-conjugated
Alternate Names AOM; ANFH; SEDC; STL1; COL11A3; Collagen alpha-1(II) chain; Alpha-1 type II collagen)

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-Fr0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25412 Rat COL2A1

Swiss-port # P05539 Rat Collagen alpha-1(II) chain

Gene Symbol COL2A1
Gene Full Name collagen, type II, alpha 1
Background This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene. [provided by RefSeq, Jul 2008]
Function Type II collagen is specific for cartilaginous tissues. It is essential for the normal embryonic development of the skeleton, for linear growth and for the ability of cartilage to resist compressive forces. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 142 kDa
PTM Probably 3-hydroxylated on prolines by LEPREL1 (By similarity). Proline residues at the third position of the tripeptide repeating unit (G-X-P) are hydroxylated in some or all of the chains. Proline residues at the second position of the tripeptide repeating unit (G-P-X) are hydroxylated in some of the chains.

The N-telopeptide is covalently linked to the helical COL2 region of alpha 1(IX), alpha 2(IX) and alpha 3(IX) chain. The C-telopeptide is covalently linked to an another site in the helical region of alpha 3(IX) COL2. [UniProt]