ARG42686

anti-Collagen IV alpha 2 antibody

anti-Collagen IV alpha 2 antibody for Western blot,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen IV alpha 2
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Hm
Tested Application ICC/IF, IHC-Fr, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen IV alpha 2
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 1697-1712 of Human Collagen IV alpha 2. (LIRTHISRCQVCMKNL)
Conjugation Un-conjugated
Alternate Names ICH; POREN2; Collagen alpha-2(IV) chain

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:200 - 1:1000
IHC-Fr1:200 - 1:1000
IHC-P1:200 - 1:1000
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Thimerosal and 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12827 Mouse COL4A2

GeneID: 1284 Human COL4A2

Swiss-port # P08122 Mouse Collagen alpha-2(IV) chain

Swiss-port # P08572 Human Collagen alpha-2(IV) chain

Gene Symbol COL4A2
Gene Full Name collagen, type IV, alpha 2
Background This gene encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. The C-terminal portion of the protein, known as canstatin, is an inhibitor of angiogenesis and tumor growth. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. [provided by RefSeq, Jul 2008]
Function Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.

Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix, basement membrane. [UniProt]
Calculated MW 168 kDa
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Type IV collagens contain numerous cysteine residues which are involved in inter- and intramolecular disulfide bonding. 12 of these, located in the NC1 domain, are conserved in all known type IV collagens.

The trimeric structure of the NC1 domains is stabilized by covalent bonds between Lys and Met residues.

Proteolytic processing produces the C-terminal NC1 peptide, canstatin. [UniProt]