ARG23685

anti-Collagen IX antibody

anti-Collagen IX antibody for ELISA,Flow cytometry,Western blot,ICC/IF,Immunohistochemistry,Immunoprecipitation and Human,Mouse,Rat,Bovine

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen IX
Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ELISA, FACS, ICC/IF, IHC, IP, WB
Specificity The antibody reacts with collagen type IX from Human, Mouse, Rat and Bovine. It exhibits no cross reactivity.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen IX
Antigen Species Rat
Immunogen Collagen type IX from Rat chondrosarcoma.
Conjugation Un-conjugated
Alternate Names MED; EDM6; STL4; DJ149L1.1.2; Collagen alpha-1(IX) chain

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACSAssay-dependent
ICC/IFAssay-dependent
IHC5 - 15 µg/ml
IP10 - 30 µg/ml
WB300 - 1200 ng/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by affinity chromatography.
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12839 Mouse COL9A1

GeneID: 1297 Human COL9A1

Swiss-port # P20849 Human Collagen alpha-1(IX) chain

Swiss-port # Q05722 Mouse Collagen alpha-1(IX) chain

Gene Symbol COL9A1
Gene Full Name collagen, type IX, alpha 1
Background This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Function Structural component of hyaline cartilage and vitreous of the eye. [UniProt]
Calculated MW 92 kDa
PTM Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. [UniProt]