ARG55560

anti-Collagen IX antibody

anti-Collagen IX antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen IX
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen IX
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 428-456 (Center) of Human Collagen IX.
Conjugation Un-conjugated
Alternate Names MED; EDM6; STL4; DJ149L1.1.2; Collagen alpha-1(IX) chain

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control MCF7

Properties

Form Liquid
Purification This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12839 Mouse COL9A1

GeneID: 1297 Human COL9A1

Swiss-port # P20849 Human Collagen alpha-1(IX) chain

Swiss-port # Q05722 Mouse Collagen alpha-1(IX) chain

Gene Symbol COL9A1
Gene Full Name collagen, type IX, alpha 1
Background This gene encodes one of the three alpha chains of type IX collagen, which is a minor (5-20%) collagen component of hyaline cartilage. Type IX collagen is usually found in tissues containing type II collagen, a fibrillar collagen. Studies in knockout mice have shown that synthesis of the alpha 1 chain is essential for assembly of type IX collagen molecules, a heterotrimeric molecule, and that lack of type IX collagen is associated with early onset osteoarthritis. Mutations in this gene are associated with osteoarthritis in humans, with multiple epiphyseal dysplasia, 6, a form of chondrodysplasia, and with Stickler syndrome, a disease characterized by ophthalmic, orofacial, articular, and auditory defects. Two transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]
Function Structural component of hyaline cartilage and vitreous of the eye. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix
Research Area Signaling Transduction antibody
Calculated MW 92 kDa
PTM Covalently linked to the telopeptides of type II collagen by lysine-derived cross-links.
Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.