ARG59478

anti-Collagen X antibody

anti-Collagen X antibody for Western blot and Human,Mouse,Pig,Rat

publication_link Publication3

Overview

Product Description Rabbit Polyclonal antibody recognizes Collagen X
Tested Reactivity Hu, Ms, Rat, Pig
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Collagen X
Antigen Species Human
Immunogen Synthetic peptide of Human Collagen X.
Conjugation Un-conjugated
Alternate Names Collagen alpha-1(X) chain

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12813 Mouse COL10A1

GeneID: 1300 Human COL10A1

Swiss-port # Q03692 Human Collagen alpha-1(X) chain

Swiss-port # Q05306 Mouse Collagen alpha-1(X) chain

Gene Symbol COL10A1
Gene Full Name collagen, type X, alpha 1
Background This gene encodes the alpha chain of type X collagen, a short chain collagen expressed by hypertrophic chondrocytes during endochondral ossification. Unlike type VIII collagen, the other short chain collagen, type X collagen is a homotrimer. Mutations in this gene are associated with Schmid type metaphyseal chondrodysplasia (SMCD) and Japanese type spondylometaphyseal dysplasia (SMD). [provided by RefSeq, Jul 2008]
Function Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. [UniProt]
Cellular Localization Secreted, extracellular space, extracellular matrix. [UniProt]
Calculated MW 66 kDa
PTM Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. [UniProt]

Specific References

Nanohydroxyapatite Hydrogel Can Promote the Proliferation and Migration of Chondrocytes and Better Repair Talar Articular Cartilage

IF / Mouse

Yuxuan Zhang et al.
Computational and Mathematical Methods in Medicine ,  (2022)

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Suramin attenuates intervertebral disc degeneration by inhibiting NF-κB signalling pathway

WB / Pig

Zi-Miao Liu et al.
Bone Joint Res.,  (2021)

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Shockwave Treatment Enhances the Extracellular Matrix Production in Articular Chondrocyte Through Activation of the ROS/MAPK/Nrf2 Signaling Pathway.

WB / Pig

Shen Po-Chih et al.
Preprint.,  (2020)

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