ARG63803

anti-Complement factor H antibody

anti-Complement factor H antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Immune System antibody

Overview

Product Description Goat Polyclonal antibody recognizes Complement factor H
Tested Reactivity Hu
Tested Application FACS, IHC-P, WB
Specificity This antibody is expected to recognize isoform a (NP_000177.2) only.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Complement factor H
Antigen Species Human
Immunogen C-HLVPDRKKDQYK
Conjugation Un-conjugated
Alternate Names Complement factor H; ARMD4; HF1; HF2; AMBP1; ARMS1; FHL1; HUS; FH; AHUS1; H factor 1; HF; CFHL3

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
IHC-P5 µg/ml
WB0.03 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human kidney and K562
Observed Size ~ 140 kDa

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3075 Human CFH

Swiss-port # P08603 Human Complement factor H

Background This gene is a member of the Regulator of Complement Activation (RCA) gene cluster and encodes a protein with twenty short consensus repeat (SCR) domains. This protein is secreted into the bloodstream and has an essential role in the regulation of complement activation, restricting this innate defense mechanism to microbial infections. Mutations in this gene have been associated with hemolytic-uremic syndrome (HUS) and chronic hypocomplementemic nephropathy. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Oct 2011]
Research Area Immune System antibody
Calculated MW 139 kDa