ARG11051

anti-Connexin 32 antibody [M12.13]

anti-Connexin 32 antibody [M12.13] for Flow cytometry,ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat

Overview

Product Description Mouse Monoclonal antibody [M12.13] recognizes Connexin 32
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone M12.13
Isotype IgG
Target Name Connexin 32
Antigen Species Rat
Immunogen Rat junctional complexes.
Epitope Located in the cytoplasmic loop.
Conjugation Un-conjugated
Alternate Names CMTX1; CX32; Gap junction beta-1 protein; CMTX; Cx32; GAP junction 28 kDa liver protein; Connexin-32

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-Fr1 - 5 µg/ml
WB0.5 - 1 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified.
Buffer PBS, 0.05% Sodium azide and 0.1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14618 Mouse GJB1

GeneID: 2705 Human GJB1

GeneID: 29584 Rat GJB1

Gene Symbol GJB1
Gene Full Name gap junction protein, beta 1, 32kDa
Background This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Oct 2008]
Function One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. [UniProt]
Calculated MW 32 kDa