ARG52893
anti-Connexin 43 antibody
anti-Connexin 43 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Connexin 43 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Connexin 43 |
| Antigen Species | Rat |
| Immunogen | Synthetic peptide derived from a segment of the 3rd cytoplasmic domain of rat connexin-43. |
| Conjugation | Un-conjugated |
| Alternate Names | Gap junction 43 kDa heart protein; CX43; PPKCA; CMDR; Gap junction alpha-1 protein; HSS; AVSD3; Connexin-43; HLHS1; EKVP; GJAL; ODDD; Cx43 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Heart, MCF-7 |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gja1 |
| Gene Full Name | gap junction protein, alpha 1 |
| Background | The connexins are a group of gap junction proteins which form a hexamer to compose a connexon. Clusters of connexons form a gap junction through which low molecular weight proteins may diffuse from cell to cell. Connexins are also thought to be essential for the development of labor contractions. |
| Function | Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm |
| Highlight | Related Antibody Duos and Panels: ARG30142 Cardiomyocyte Cell Surface Marker Antibody Panel (Cx43, Desmin, SIRP) Related products: Connexin43 antibodies; Connexin43 Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Molecular mechanisms of labor initiation found |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody |
| Calculated MW | 43 kDa |
| PTM | Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity). Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. |
Customer's Feedback
Excellent
Review for anti-Connexin 43 antibody
Application：WB
Sample：MCF7 and Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:30
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
