ARG52251
anti-Connexin 43 phospho (Ser368) antibody
anti-Connexin 43 phospho (Ser368) antibody for Western blot and Rat
Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Connexin 43 phospho (Ser368) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, Chk, Dog, Gpig, Zfsh |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Connexin 43 |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser368 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | Gap junction 43 kDa heart protein; CX43; PPKCA; CMDR; Gap junction alpha-1 protein; HSS; AVSD3; Connexin-43; HLHS1; EKVP; GJAL; ODDD; Cx43 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~43k connexin43 protein phosphorylated at Ser368. Immunolabeling is blocked by λ-phosphatase treatment. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GJA1 |
| Gene Full Name | gap junction protein, alpha 1 |
| Background | Gap junctional intercellular communication is thought to play a key role in development and may also be involved in epilepsy (Aronica et al., 2001). Connexin43 forms gap-junctional channels and regulates the permeability of these gap junctions to small organic molecules. Permeability of connexin43 is known to be regulated by phosphorylation at Ser368 by protein kinase C (Yogo et al., 2002; Bao et al., 2004a). Phosphorylation of Ser368 by PKC induces a conformational change of connexin43 that results in a decrease in gap junction permeability (Bao et al., 2004b). |
| Research Area | Cell Biology and Cellular Response antibody; Signaling Transduction antibody; Cardiomyocyte Cell Surface Marker antibody |
| Calculated MW | 43 kDa |
| PTM | Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity). Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2. S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication. |
