ARG54108
anti-CrkII antibody
anti-CrkII antibody for Western blot and Human
Cancer antibody; Signaling Transduction antibody
Overview
Product Description | Mouse Monoclonal antibody recognizes CRK |
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Tested Reactivity | Hu |
Tested Application | WB |
Host | Mouse |
Clonality | Monoclonal |
Isotype | IgG2b |
Target Name | CrkII |
Antigen Species | Human |
Immunogen | Purified recombinant human CrkII protein fragments expressed in E.coli. |
Conjugation | Un-conjugated |
Alternate Names | CRKII; p38; Proto-oncogene c-Crk; Adapter molecule crk |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||
Observed Size | 34 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified |
Buffer | 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol |
Preservative | 0.2% Sodium azide |
Stabilizer | 50% Glycerol |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | CRK |
Gene Full Name | v-crk avian sarcoma virus CT10 oncogene homolog |
Background | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. |
Function | The Crk-I and Crk-II forms differ in their biological activities. Crk-II has less transforming activity than Crk-I. Crk-II mediates attachment-induced MAPK8 activation, membrane ruffling and cell motility in a Rac-dependent manner. Involved in phagocytosis of apoptotic cells and cell motility via its interaction with DOCK1 and DOCK4. May regulate the EFNA5-EPHA3 signaling. [UniProt] |
Cellular Localization | Cytoplasm, Cell membrane |
Research Area | Cancer antibody; Signaling Transduction antibody |
Calculated MW | 34 kDa |
PTM | Phosphorylation of Crk-II (40 kDa) gives rise to a 42 kDa form. Isoform Crk-II is phosphorylated by KIT. Phosphorylated on Tyr-221 upon cell adhesion. Results in the negative regulation of the association with SH2- and SH3-binding partners, possibly by the formation of an intramolecular interaction of phosphorylated Tyr-221 with the SH2 domain. This leads finally to the down-regulation of the Crk signaling pathway. Proline isomerization at Pro-237 by PPIA acts as a switch between two conformations: an autoinhibitory conformation in the cis form, where the tandem SH3 domains interact intramolecularly, and an activated conformation in the trans form. |