ARG59155

anti-Cullin 5 antibody

anti-Cullin 5 antibody for ICC/IF,Western blot and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Cullin 5
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cullin 5
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-300 of Human Cullin 5 (NP_003469.2).
Conjugation Un-conjugated
Alternate Names VACM-1; CUL-5; VACM1; Vasopressin-activated calcium-mobilizing receptor 1; Cullin-5

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control PC3
Observed Size 98 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 75717 Mouse CUL5

GeneID: 8065 Human CUL5

Swiss-port # Q93034 Human Cullin-5

Swiss-port # Q9D5V5 Mouse Cullin-5

Gene Symbol CUL5
Gene Full Name cullin 5
Function Core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAK2. Seems to be involved in proteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein. May form a cell surface vasopressin receptor. [UniProt]
Calculated MW 91 kDa
PTM Neddylated; which enhances the ubiquitination activity of SCF and prevents binding of the inhibitor CAND1. Deneddylated via its interaction with the COP9 signalosome (CSN). [UniProt]