ARG55257

anti-Cyclin B1 antibody

anti-Cyclin B1 antibody for ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Human,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Cyclin B1

Tested Reactivity Hu, Rat
Tested Application ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cyclin B1
Antigen Species Human
Immunogen Synthetic peptide of Human Cyclin B1 (NP_114172.1).
Conjugation Un-conjugated
Alternate Names G2/mitotic-specific cyclin-B1; CCNB

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC1:50 - 1:200
IP1:20 - 1:100
WB1:500 - 1:3000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25203 Rat CCNB1

GeneID: 891 Human CCNB1

Swiss-port # P14635 Human G2/mitotic-specific cyclin-B1

Swiss-port # P30277 Rat G2/mitotic-specific cyclin-B1

Gene Symbol CCNB1
Gene Full Name cyclin B1
Background The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]
Function Essential for the control of the cell cycle at the G2/M (mitosis) transition. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Calculated MW 48 kDa
PTM Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases.
Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site.

Customer's Feedback

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Review for anti-Cyclin B1 antibody

Application:WB

Sample:Rat lung

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Good

Review for anti-Cyclin B1 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:2000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

A novel mechanism driving poor-prognostic gastric cancer: overexpression of the transcription factor Krüppel-like factor 16 promotes growth and metastasis of gastric cancer through regulating the Notch pathway.

WB / Human

Ding-Ping Sun et al.
Am J Cancer Res.,  (2021)

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Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.

WB / Human

Yang Yan et al.
Cancer Cell Int.,  (2020)

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