ARG62456
anti-Cyclin B1 antibody [V152]
anti-Cyclin B1 antibody [V152] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Overview
Product Description | Mouse Monoclonal antibody [V152] recognizes Cyclin B1 |
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Tested Reactivity | Hu |
Predict Reactivity | Ms |
Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
Specificity | Cyclin B1 expression is restricted to a specific short period of the cell cycle with cyclin B1 expression detected earlier and peaking in concentration before cyclin B2 expression. |
Host | Mouse |
Clonality | Monoclonal |
Clone | V152 |
Isotype | IgG1 |
Target Name | Cyclin B1 |
Antigen Species | Hamster |
Immunogen | His-tagged Hamster Cyclin B1 expressed in bacteria |
Conjugation | Un-conjugated |
Alternate Names | G2/mitotic-specific cyclin-B1; CCNB |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Protein G purified |
Buffer | PBS (pH 7.4) and 0.02% Sodium azide |
Preservative | 0.02% Sodium azide |
Concentration | 0.2 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | Ccnb1 |
Gene Full Name | cyclin B1 |
Background | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008] |
Highlight | Related Antibody Duos and Panels: ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) Related products: Cyclin B1 antibodies; Cyclin B1 Duos / Panels; Anti-Mouse IgG secondary antibodies; |
Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
Calculated MW | 48 kDa |
PTM | Ubiquitinated by the SCF(NIPA) complex during interphase, leading to its destruction. Not ubiquitinated during G2/M phases. Phosphorylated by PLK1 at Ser-133 on centrosomes during prophase: phosphorylation by PLK1 does not cause nuclear import. Phosphorylation at Ser-147 was also reported to be mediated by PLK1 but Ser-133 seems to be the primary phosphorylation site. |
Clone References