ARG52923
anti-Cyclin D1 antibody [SP4]
anti-Cyclin D1 antibody [SP4] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody
Publication4
Overview
| Product Description | Rabbit Monoclonal antibody [SP4] recognizes Cyclin D1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Dog |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP4 |
| Isotype | IgG |
| Target Name | Cyclin D1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from C-terminus of human cyclin D1. |
| Conjugation | Un-conjugated |
| Alternate Names | B-cell lymphoma 1 protein; PRAD1; U21B31; D11S287E; BCL-1; G1/S-specific cyclin-D1; BCL-1 oncogene; BCL1; PRAD1 oncogene |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Mantle Cell Lymphoma, Tonsil, MCF-7 |
Properties
| Form | Liquid |
|---|---|
| Buffer | Tissue culture supernatant in TBS (pH 7.5), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Cyclin D1 or PRAD-1 or bcl-1 is one of the key cell cycle regulators, and functions in association with cdk4 and / or cdk6 by phosphorylating the Rb protein. It is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). |
| Cellular Localization | Nucleus |
| Highlight | Related products: Cyclin D1 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Lymphoma |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody |
| Calculated MW | 34 kDa |
| PTM | Phosphorylation at Thr-286 by MAP kinases is required for ubiquitination and degradation following DNA damage. It probably plays an essential role for recognition by the FBXO31 component of SCF (SKP1-cullin-F-box) protein ligase complex. Ubiquitinated, primarily as 'Lys-48'-linked polyubiquitination. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex containing FBXO4 and CRYAB. Following DNA damage it is ubiquitinated by some SCF (SKP1-cullin-F-box) protein ligase complex containing FBXO31. SCF-type ubiquitination is dependent on Thr-286 phosphorylation (By similarity). Ubiquitinated also by UHRF2 apparently in a phosphorylation-independent manner. Ubiquitination leads to its degradation and G1 arrest. Deubiquitinated by USP2; leading to its stabilization. |
Customer's Feedback
Excellent
Review for anti-Cyclin D1 antibody [SP4]
Application：WB
Sample：Rat kidney
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
A combined bioinformatics and experimental approach identifies RMI2 as a Wnt/β-catenin signaling target gene related to hepatocellular carcinoma
WB / Human
Multilevel Regulation of β-Catenin Activity by SETD2 Suppresses the Transition from Polycystic Kidney Disease to Clear Cell Renal Cell Carcinoma
WB / Mouse
SETD2 deficiency impairs β-catenin destruction complex to facilitate renal cell carcinoma formation.
WB, IHC-P / Mouse
ERK Kinases Phosphorylate Lin28a to Modulate P19 Cell Proliferation and Differentiation
WB / Mouse
