ARG52927
anti-Cyclin E1 antibody
anti-Cyclin E1 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cyclin E1 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Pig |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cyclin E1 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide from C-terminus of human cyclin E. |
| Conjugation | Un-conjugated |
| Alternate Names | pCCNE1; CCNE; G1/S-specific cyclin-E1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 10 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Breast Carcinoma, Placenta |
Properties
| Form | Liquid |
|---|---|
| Purification | Immunogen affinity purified |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analysis indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers. |
| Cellular Localization | Nucleus |
| Highlight | Related Antibody Duos and Panels: ARG30139 Cell Cycle Marker Antibody Panel (Cyclin B1, Cyclin E) Related products: Cyclin E1 antibodies; Cyclin E1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Cell Cycle Study antibody |
| Calculated MW | 47 kDa |
| PTM | Phosphorylation of both Thr-395 by GSK3 and Ser-399 by CDK2 creates a high affinity degron recognized by FBXW7, and accelerates degradation via the ubiquitin proteasome pathway. Phosphorylation at Thr-77 creates a low affinity degron also recognized by FBXW7. Ubiquitinated by UHRF2; appears to occur independently of phosphorylation. |
