ARG62461

anti-Cytochrome C antibody [6H2.B4]

anti-Cytochrome C antibody [6H2.B4] for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
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Overview

Product Description

Mouse Monoclonal antibody [6H2.B4] recognizes Cytochrome C

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IP, WB
Host Mouse
Clonality Monoclonal
Clone 6H2.B4
Isotype IgG1, kappa
Target Name Cytochrome C
Antigen Species Rat
Immunogen Rat cyt c-OVA
Conjugation Un-conjugated
Alternate Names CYC; HCS; Cytochrome c; THC4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 µg per 10^6 cells
ICC/IFAssay-dependent
IP2-4 µg /1 x10^7 cell
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification affinity chromatography
Buffer PBS (pH 7.2) and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13063 Mouse CYCS

GeneID: 25309 Rat CYCS

GeneID: 54205 Human CYCS

Gene Symbol Cycs
Gene Full Name cytochrome c, somatic
Background This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity). [UniProt]
Cellular Localization Mitochondrial intermembrane space
Highlight Related Antibody Duos and Panels:
ARG30271 Mitochondrial Marker Antibody Panel (Cytochrome C, COX4, HSP60)
ARG30276 Cytochrome-C fractionation Antibody Panel (Cytochrome-C, COX IV, beta Actin)
Related products:
Cytochrome C antibodies; Cytochrome C Duos / Panels; Anti-Mouse IgG secondary antibodies;
Related news:
Understanding Your Cells: Choose the right markers
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 12 kDa
PTM Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

Customer's Feedback

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Review for anti-Cytochrome C antibody [6H2.B4]

Application:WB

Sample:Mouse kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

nuts_pic      Excellent

Review for anti-Cytochrome C antibody [6H2.B4]

Application:WB

Sample:Rat kidney lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC