ARG56070

anti-Cytochrome C antibody [7H8.2C12]

anti-Cytochrome C antibody [7H8.2C12] for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

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Overview

Product Description

Mouse Monoclonal antibody [7H8.2C12] recognizes Cytochrome C

Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone 7H8.2C12
Isotype IgG2b, kappa
Target Name Cytochrome C
Immunogen Synthetic peptides around aa. 1-80, 81-104 and 66-104 of Pigeon Cytochrome C.
Conjugation Un-conjugated
Alternate Names CYC; HCS; Cytochrome c; THC4

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 2 µg/10^6 cells
IHC-P2 - 5 µg/ml
WB1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10 mM Citrate buffer (pH 6.0) for 10-20 min, followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 13063 Mouse CYCS

GeneID: 25309 Rat CYCS

GeneID: 54205 Human CYCS

Gene Symbol CYCS
Gene Full Name cytochrome c, somatic
Background This gene encodes a small heme protein that functions as a central component of the electron transport chain in mitochondria. The encoded protein associates with the inner membrane of the mitochondrion where it accepts electrons from cytochrome b and transfers them to the cytochrome oxidase complex. This protein is also involved in initiation of apoptosis. Mutations in this gene are associated with autosomal dominant nonsyndromic thrombocytopenia. Numerous processed pseudogenes of this gene are found throughout the human genome.[provided by RefSeq, Jul 2010]
Function Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.

Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases. [UniProt]
Cellular Localization Cytoplasmic
Calculated MW 12 kDa
PTM Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.

Customer's Feedback

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Review for anti-Cytochrome C antibody [7H8.2C12]

Application:WB

Sample:Mouse stomach

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:200

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC