ARG62970
anti-Cytokeratin 10 antibody [VIK-10]
anti-Cytokeratin 10 antibody [VIK-10] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,ICC/IF and Human
Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [VIK-10] recognizes Cytokeratin 10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, IP |
| Specificity | The clone VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | VIK-10 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 10 |
| Antigen Species | Human |
| Immunogen | Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction. |
| Conjugation | Un-conjugated |
| Alternate Names | KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Pretreatment: Trypsin, at 37°C. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | IHC-P: Skin |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from ascites by protein-A affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT10 |
| Gene Full Name | keratin 10, type I |
| Background | Cytokeratin 10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin. (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization. (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt] |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 59 kDa |
