ARG62970

anti-Cytokeratin 10 antibody [VIK-10]

anti-Cytokeratin 10 antibody [VIK-10] for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,ICC/IF and Human

Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [VIK-10] recognizes Cytokeratin 10
Tested Reactivity Hu
Tested Application ICC/IF, IHC-P, IP
Specificity The clone VIK-10 reacts with Cytokeratin 10 (56.5 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone VIK-10
Isotype IgG1
Target Name Cytokeratin 10
Antigen Species Human
Immunogen Cytoskeleton preparation extracted from human epidermis by detergent/high salt extraction.
Conjugation Un-conjugated
Alternate Names KPP; K10; CK-10; BIE; Keratin, type I cytoskeletal 10; Cytokeratin-10; CK10; BCIE; Keratin-10; EHK

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
Application Note IHC-P: Pretreatment: Trypsin, at 37°C.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Skin

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3858 Human KRT10

Swiss-port # P13645 Human Keratin, type I cytoskeletal 10

Gene Symbol KRT10
Gene Full Name keratin 10, type I
Background Cytokeratin 10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins. Keratins are heteropolymeric structural proteins which form the intermediate filament. These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells. Mutations in this gene are associated with epidermolytic hyperkeratosis. This gene is located within a cluster of keratin family members on chromosome 17q21. [provided by RefSeq, Jul 2008]
Function Cytokeratin 10 plays a role in the establishment of the epidermal barrier on plantar skin.

(Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization.

(Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur. [UniProt]
Research Area Signaling Transduction antibody
Calculated MW 59 kDa