ARG55255

anti-Cytokeratin 12 antibody

anti-Cytokeratin 12 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 12
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 12
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide corresponding to aa. 442-471 (C-terminus) of Human Cytokeratin 12.
Conjugation Un-conjugated
Alternate Names K12; CK-12; Cytokeratin-12; Keratin-12; Keratin, type I cytoskeletal 12

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:10 - 1:50
IHC-P1:10 - 1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse eyeball

Properties

Form Liquid
Purification Purification with Protein A and immunogen peptide
Buffer PBS and 0.09% (W/V) Sodium azide
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 360625 Rat KRT12

GeneID: 3859 Human KRT12

Swiss-port # Q6IFW5 Rat Keratin, type I cytoskeletal 12

Swiss-port # Q99456 Human Keratin, type I cytoskeletal 12

Gene Symbol KRT12
Gene Full Name keratin 12, type I
Background KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
Function May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity (By similarity). [UniProt]
Research Area Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 54 kDa