ARG66971
anti-Cytokeratin 14 + 17 antibody
anti-Cytokeratin 14 + 17 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Cytokeratin 14 + 17 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Cytokeratin 14 + 17 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the middle region of Human Cytokeratin 14. |
| Conjugation | Un-conjugated |
| Alternate Names | K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14; KRT17; Keratin 17; Keratin, Type I Cytoskeletal 17; Keratin 17, Type I; Cytokeratin-17; CK-17; PCHC1; K17; Keratin-17; PC2; PC |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | MDA-MB-231; Colorectal carcinoma; Breast carcinoma | ||||||||
| Observed Size | ~ 45 - 55 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300, 20% Glycerol and 3% BSA. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol and 3% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT14 + KRT17 |
| Gene Full Name | keratin 14 + 17, type I |
| Background | Cytokeratin 14 encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008] |
| Function | The nonhelical tail domain of Cytokeratin 14 is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern. [UniProt] |
| Calculated MW | 52 kDa |
| PTM | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. [UniProt] |
