ARG40225

anti-Cytokeratin 14 antibody

anti-Cytokeratin 14 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 14
Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 14
Antigen Species Human
Immunogen Synthetic peptide derived from Human Cytokeratin 14.
Conjugation Un-conjugated
Alternate Names K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A431
Observed Size ~ 45 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3861 Human KRT14

Swiss-port # P02533 Human Keratin, type I cytoskeletal 14

Gene Symbol KRT14
Gene Full Name keratin 14, type I
Background This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Note=Expressed in both as a filamentous pattern. [UniProt]
Calculated MW 52 kDa
PTM A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.

Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. [UniProt]