ARG55964

anti-Cytokeratin 14 antibody [LL002]

anti-Cytokeratin 14 antibody [LL002] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Dog,Elephant,Human,Mouse,Pig,Rat

Overview

Product Description Mouse Monoclonal antibody [LL002] recognizes Cytokeratin 14
Tested Reactivity Hu, Ms, Rat, Dog, Elp, Pig
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone LL002
Isotype IgG3
Target Name Cytokeratin 14
Antigen Species Human
Immunogen A 15-mer synthetic peptide from the C-terminus of Human Cytokeratin 14.
Conjugation Un-conjugated
Alternate Names K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-Fr1:20 - 300
IHC-P1 - 2 µg/ml
WB0.5 - 1 µg/ml
Application Note Antigen retrieval for IHC-P: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 53 kDa

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.05% Sodium azide and 0.1 mg/ml BSA
Preservative 0.05% Sodium azide
Stabilizer 0.1 mg/ml BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16664 Mouse KRT14

GeneID: 287701 Rat KRT14

GeneID: 3861 Human KRT14

Gene Symbol KRT14
Gene Full Name keratin 14, type I
Background This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]
Function The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro. [UniProt]
Cellular Localization Cytoplasmic
Calculated MW 52 kDa
PTM A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus.
Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex.