ARG53180
anti-Cytokeratin 14 antibody [SP53]
anti-Cytokeratin 14 antibody [SP53] for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human
Signaling Transduction antibody
Overview
| Product Description | Rabbit Monoclonal antibody [SP53] recognizes Cytokeratin 14 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SP53 |
| Isotype | IgG |
| Target Name | Cytokeratin 14 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to C-terminus of Human Cytokeratin 14. |
| Conjugation | Un-conjugated |
| Alternate Names | K14; CK-14; Cytokeratin-14; Keratin, type I cytoskeletal 14; EBS3; EBS4; NFJ; Keratin-14; CK14 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min. Incubation Time: 30 min at RT. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Prostate, Skin, Squamous Cell Carcinoma, A431 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by protein A/G |
| Buffer | PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide |
| Preservative | < 0.1% Sodium azide |
| Stabilizer | 1% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | Keratin 14 belongs to the type A (acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5. |
| Cellular Localization | Cytoplasm |
| Highlight | Related products: Keratin14 antibodies; Anti-Rabbit IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients |
| Research Area | Signaling Transduction antibody |
| Calculated MW | 52 kDa |
| PTM | A disulfide bond is formed between rather than within filaments and promotes the formation of a keratin filament cage around the nucleus. Ubiquitinated by the BCR(KLHL24) E3 ubiquitin ligase complex. |
