ARG62526

anti-Cytokeratin 15 antibody [LHK15]

anti-Cytokeratin 15 antibody [LHK15] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,SDS-PAGE,Western blot and Cow,Human,Mouse,Rat

Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [LHK15] recognizes Cytokeratin 15
Tested Reactivity Hu, Ms, Rat, Cow
Tested Application FACS, ICC/IF, IHC-P, SDS-PAGE, WB
Host Mouse
Clonality Monoclonal
Clone LHK15
Isotype IgG2a, kappa
Target Name Cytokeratin 15
Antigen Species Human
Immunogen A 17-mer synthetic peptide from the C-terminus of Human Cytokeratin 15.
Conjugation Un-conjugated
Alternate Names CK-15; K15; Keratin, type I cytoskeletal 15; Cytokeratin-15; Keratin-15; K1CO; CK15

Application Instructions

Application Suggestion
Tested Application Dilution
FACS0.5 - 1 µg/10^6 cells
ICC/IF1 - 2 µg/ml
IHC-P0.5 - 1 µg/ml
SDS-PAGEAssay-dependent
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS, 1% BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 1% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16665 Mouse KRT15

GeneID: 287700 Rat KRT15

GeneID: 3866 Human KRT15

Gene Symbol KRT15
Gene Full Name keratin 15, type I
Background The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 49 kDa

Clone References

Aquaporin 1-positive stromal niche-like cells directly interact with N-cadherin-positive clusters in the basal limbal epithelium.

IHC / Human

Higa K et al.
Stem Cell Res.,  (2013)

publication_link

 

hr_line

Intraepithelial paracrine Hedgehog signaling induces the expansion of ciliated cells that express diverse progenitor cell markers in the basal epithelium of the mouse mammary gland.

IHC / Mouse

García-Zaragoza E et al.
Dev Biol.,  (2012)

publication_link

 

hr_line

Patched1 inhibits epidermal progenitor cell expansion and basal cell carcinoma formation by limiting Igfbp2 activity.

IHC-P / Mouse

Villani RM et al.
Cancer Prev Res (Phila).,  (2010)

publication_link

 

hr_line

Human hair follicle pluripotent stem (hfPS) cells promote regeneration of peripheral-nerve injury: an advantageous alternative to ES and iPS cells.

ICC/IF / Human

Amoh Y et al.
J Cell Biochem.,  (2009)

publication_link

 

hr_line

Identification of a subset of breast carcinomas characterized by expression of cytokeratin 15: relationship between CK15+ progenitor/amplified cells and pre-malignant lesions and invasive disease.

WB / Human

Celis JE et al.
Mol Oncol.,  (2007)

publication_link

 

hr_line

Isolation and identification of stem cells from adult cashmere goat skin.

ICC/IF / Ovine*

Liu Y et al.
Int J Dermatol.,  (2008)

publication_link

 

hr_line