ARG62975

anti-Cytokeratin 18 antibody [C-04]

anti-Cytokeratin 18 antibody [C-04] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Cat,Cow,Dog,Goat,Hamster,Horse,Human,Mammal,Mouse,Pig,Rat,Sheep

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [C-04] recognizes Cytokeratin 18

Tested Reactivity Hu, Ms, Rat, Cat, Cow, Dog, Goat, Hm, Hrs, Mamm, Pig, Sheep
Tested Application ELISA, FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone C-04 reacts with Cytokeratin 18 (45 kDa), a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-04
Isotype IgG1
Target Name Cytokeratin 18
Immunogen Cytoskeleton preparation of epidermal carcinoma cell line A431.
Conjugation Un-conjugated
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 4 µg/ml
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WB1 - 2 µg/ml
Application Note Western Blotting: incubate overnight in 4°C. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing conditions. SDS-PAGE (10% separating gel).
ELISA: Application note: The antibody C-04 has been tested as the capture antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with antibody DA-7 (cat. no. ARG62977) and with the antibody DC-10 (cat. no. ARG53186).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: HeLa and MCF-7
IHC-P: Colon and kidney tissue.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16668 Mouse KRT18

GeneID: 294853 Rat KRT18

GeneID: 3875 Human KRT18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Clone References

TP53 supports basal-like differentiation of mammary epithelial cells by preventing translocation of deltaNp63 into nucleoli.

ICC/IF / Human

Munne PM et al.
Sci Rep.,  (2014)

publication_link

 

hr_line

Development of hepatocellular carcinoma in a murine model of nonalcoholic steatohepatitis induced by use of a high-fat/fructose diet and sedentary lifestyle.

IHC / Mouse

Dowman JK et al.
Am J Pathol.,  (2014)

publication_link

 

hr_line

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland.

IHC-P / Mouse

Llobet-Navas D et al.
Genes Dev.,  (2014)

publication_link

 

hr_line

Miz1 deficiency in the mammary gland causes a lactation defect by attenuated Stat5 expression and phosphorylation.

WB / Mouse

Sanz-Moreno A et al.
PLoS One.,  (2014)

publication_link

 

hr_line

Salisphere derived c-Kit+ cell transplantation restores tissue homeostasis in irradiated salivary gland.

IHC-Fr / Mouse

Nanduri LS et al.
Radiother Oncol.,  (2013)

publication_link

 

hr_line

Feasibility of fast dynamic MRI for noninvasive monitoring during ectopic liver cell transplantation to the spleen in a porcine model.

IHC / Pig

Raschzok N et al.
AJR Am J Roentgenol.,  (2012)

publication_link

 

hr_line

Conditional activation of Pik3ca(H1047R) in a knock-in mouse model promotes mammary tumorigenesis and emergence of mutations.

ICC/IF / Mouse

Yuan W et al.
Oncogene.,  (2013)

publication_link

 

hr_line

Stemness markers characterize IGR-CaP1, a new cell line derived from primary epithelial prostate cancer.

FACS / Human

Chauchereau A et al.
Exp Cell Res.,  (2011)

publication_link

 

hr_line

Suppression of keratin 18 gene expression in bovine blastocysts by RNA interference.

ICC/IF / Cow

Goossens K et al.
Reprod Fertil Dev.,  (2010)

publication_link

 

hr_line

Generation of a prostate from a single adult stem cell.

FACS, IHC-Fr / Mouse

Leong KG et al.
Nature.,  (2008)

publication_link

 

hr_line