ARG62977

anti-Cytokeratin 18 antibody [DA-7]

anti-Cytokeratin 18 antibody [DA-7] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [DA-7] recognizes Cytokeratin 18

Tested Reactivity Hu
Species Does Not React With Ms, Rat, Bov, Dog, Hm, Pig, Sheep
Tested Application ELISA, ICC/IF, IHC-P, IP, WB
Specificity The clone DA-7 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone DA-7
Isotype IgG1
Target Name Cytokeratin 18
Antigen Species Human
Immunogen Human breast carcinoma cell line PMC-42.
Conjugation Un-conjugated
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WB1 - 2 µg/ml
Application Note WB: Incubation: Overnight at 4°C. Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer. Application note: Non-reducing condition. SDS-PAGE (10% separating gel).
ELISA: The clone DA-7 has been tested as the detection antibody in a sandwich ELISA for analysis of Cytokeratin 18 in combination with the clone C-04 (ARG62975).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: HeLa, A431 and MCF7
IHC-P: Colon and skin
ICC/IF: HeLa and MCF7
ELISA: MCF7

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3875 Human KRT18

Swiss-port # P05783 Human Keratin, type I cytoskeletal 18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Clone References

In vitro hepatic differentiation of human bone marrow mesenchymal stem cells under differential exposure to liver-specific factors.

ICC/IF / Human

Chivu M et al.
Transl Res.,  (2009)

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Quantitative multiplexed quantum dot immunohistochemistry.

ICC/IF / Human

Sweeney E et al.
Biochem Biophys Res Commun.,  (2008)

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Culture in vitro modulation of human leukocyte antigen molecules and costimulatory molecules on human retinal pigment epithelium.

IHC / Human

Xie MS et al.
Ophthalmologica.,  (2008)

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