ARG53927

anti-Cytokeratin 18 antibody [DC 10] (FITC)

anti-Cytokeratin 18 antibody [DC 10] (FITC) for Flow cytometry and Human

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description FITC-conjugated Mouse Monoclonal antibody [DC 10] recognizes Cytokeratin 18
Tested Reactivity Hu
Species Does Not React With Ms, Rat, Bov, Dog, Hm, Pig, Sheep
Tested Application FACS
Specificity The clone DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone DC 10
Isotype IgG1
Target Name Cytokeratin 18
Antigen Species Human
Immunogen Human breast carcinoma cell line PMC-42.
Conjugation FITC
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3875 Human KRT18

Swiss-port # P05783 Human Keratin, type I cytoskeletal 18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Clone References

Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.

WB / Mouse

Snider NT et al.
J Biol Chem.,  (2011)

publication_link

 

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Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.

IHC / Human

Robertson FM et al.
J Biomol Screen.,  (2010)

publication_link

 

hr_line

Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.

WB / Human

Yalcin-Ozuysal O et al.
Cell Death Differ.,  (2010)

publication_link

 

hr_line

Endometrial profile of tamoxifen and low-dose estradiol combination therapy.

IHC / Primates

Wood CE et al.
Clin Cancer Res.,  (2010)

publication_link

 

hr_line

Directed differentiation of human embryonic stem cells to epidermal progenitors.

FACS / Human

Metallo CM et al.
Methods Mol Biol.,  (2010)

publication_link

 

hr_line

Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.

WB / Human

Chang CY et al.
Res Vet Sci.,  (2010)

publication_link

 

hr_line

Mouse mammary tumor virus p75 and p110 CUX1 transgenic mice develop mammary tumors of various histologic types.

WB / Mouse

Cadieux C et al.
Cancer Res.,  (2009)

publication_link

 

hr_line

Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.

ICC/IF / Human

Sumitran-Holgersson S et al.
Cell Transplant.,  (2009)

publication_link

 

hr_line

Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.

ELISA / Human

Tao GZ et al.
J Pathol.,  (2008)

publication_link

 

hr_line

Mammary gland development in early pubertal female macaques.

IHC / Rhesus Monkey

Wood CE et al.
Toxicol Pathol.,  (2007)

publication_link

 

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Epithelial phenotypes in the developing human prostate.

IHC-P / Human

Letellier G et al.
J Histochem Cytochem.,  (2007)

publication_link

 

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