ARG53927
anti-Cytokeratin 18 antibody [DC 10] (FITC)
anti-Cytokeratin 18 antibody [DC 10] (FITC) for Flow cytometry and Human
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | FITC-conjugated Mouse Monoclonal antibody [DC 10] recognizes Cytokeratin 18 |
|---|---|
| Tested Reactivity | Hu |
| Species Does Not React With | Ms, Rat, Bov, Dog, Hm, Pig, Sheep |
| Tested Application | FACS |
| Specificity | The clone DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | DC 10 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 18 |
| Antigen Species | Human |
| Immunogen | Human breast carcinoma cell line PMC-42. |
| Conjugation | FITC |
| Alternate Names | Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification Note | The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC. |
| Buffer | PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA |
| Preservative | 15 mM Sodium azide |
| Stabilizer | 0.2% (w/v) high-grade protease free BSA |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT18 |
| Gene Full Name | keratin 18, type I |
| Background | Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 48 kDa |
| PTM | Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6. Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7. O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation. |
Clone References
Keratin hypersumoylation alters filament dynamics and is a marker for human liver disease and keratin mutation.
WB / Mouse
Imaging and analysis of 3D tumor spheroids enriched for a cancer stem cell phenotype.
IHC / Human
Antagonistic roles of Notch and p63 in controlling mammary epithelial cell fates.
WB / Human
Endometrial profile of tamoxifen and low-dose estradiol combination therapy.
IHC / Primates
Directed differentiation of human embryonic stem cells to epidermal progenitors.
FACS / Human
Assessment of the tumorigenesis and drug susceptibility of three new canine mammary tumor cell lines.
WB / Human
Mouse mammary tumor virus p75 and p110 CUX1 transgenic mice develop mammary tumors of various histologic types.
WB / Mouse
Generation of hepatocyte-like cells from in vitro transdifferentiated human fetal pancreas.
ICC/IF / Human
Monitoring of epithelial cell caspase activation via detection of durable keratin fragment formation.
ELISA / Human
Mammary gland development in early pubertal female macaques.
IHC / Rhesus Monkey
Epithelial phenotypes in the developing human prostate.
IHC-P / Human
