ARG62973

anti-Cytokeratin 18 antibody [DC10] (Biotin)

anti-Cytokeratin 18 antibody [DC10] (Biotin) for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Controls and Markers antibody; Signaling Transduction antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [DC10] recognizes Cytokeratin 18
Tested Reactivity Hu
Species Does Not React With Ms, Rat, Bov, Dog, Hm, Pig, Sheep
Tested Application ELISA, FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone DC-10 reacts with Cytokeratin 18 (45 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone DC10
Isotype IgG1
Target Name Cytokeratin 18
Antigen Species Human
Immunogen Human breast carcinoma cell line PMC-42.
Conjugation Biotin
Alternate Names Keratin, type I cytoskeletal 18; Cytokeratin-18; K18; CK-18; Cell proliferation-inducing gene 46 protein; Keratin-18; CYK18

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS2 - 6 µg/ml
ICC/IFAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT-29
Observed Size ~ 43 kDa

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3875 Human KRT18

Swiss-port # P05783 Human Keratin, type I cytoskeletal 18

Gene Symbol KRT18
Gene Full Name keratin 18, type I
Background Cytokeratin 18, together with its filament partner Cytokeratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Function Cytokeratin 18 involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection. [UniProt]
Research Area Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylation at Ser-34 increases during mitosis. Hyperphosphorylated at Ser-53 in diseased cirrhosis liver. Phosphorylation increases by IL-6.
Proteolytically cleaved by caspases during epithelial cell apoptosis. Cleavage occurs at Asp-238 by either caspase-3, caspase-6 or caspase-7.
O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.

Clone References

Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule.

Lauerová L et al.
Hybridoma.,  (1988)

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