ARG53929

anti-Cytokeratin 19 antibody [A53-B/A2] (Biotin)

anti-Cytokeratin 19 antibody [A53-B/A2] (Biotin) for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Biotin-conjugated Mouse Monoclonal antibody [A53-B/A2] recognizes Cytokeratin 19
Tested Reactivity Hu
Tested Application ELISA, FACS, ICC/IF, IHC-P, IP, WB
Specificity The clone A53-B/A2 reacts with Rod domain of cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Host Mouse
Clonality Monoclonal
Clone A53-B/A2
Isotype IgG2a
Target Name Cytokeratin 19
Antigen Species Human
Immunogen MCF-7 human breast adenocarcinoma cell line
Conjugation Biotin
Alternate Names Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
FACS1 - 5 µg/ml
ICC/IF1:500
IHC-PAssay-dependent
IPAssay-dependent
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HT-29
Observed Size ~ 40 kDa

Properties

Form Liquid
Purification Note The purified antibody is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3880 Human KRT19

Swiss-port # P08727 Human Keratin, type I cytoskeletal 19

Gene Symbol KRT19
Gene Full Name keratin 19, type I
Background Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Highlight Related products:
Cytokeratin 19 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Therapeutic strategies against PDAC
Research Area Signaling Transduction antibody
Calculated MW 44 kDa