ARG40282

anti-Cytokeratin 19 antibody

anti-Cytokeratin 19 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 19
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 19
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 334-372 of Human Cytokeratin 19. (QLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSR)
Conjugation Un-conjugated
Alternate Names Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P0.5 - 1 µg/ml
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min, or performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 44 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16669 Mouse KRT19

GeneID: 360626 Rat KRT19

GeneID: 3880 Human KRT19

Gene Symbol KRT19
Gene Full Name keratin 19, type I
Background Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
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Cytokeratin 19 antibodies; Anti-Rabbit IgG secondary antibodies;
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Calculated MW 44 kDa