ARG62978

anti-Cytokeratin 19 antibody [BA-17]

anti-Cytokeratin 19 antibody [BA-17] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Human

Signaling Transduction antibody
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Overview

Product Description

Mouse Monoclonal antibody [BA-17] recognizes Cytokeratin 19

Tested Reactivity Hu
Predict Reactivity Ms
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone BA-17 reacts with cytokeratin 19 (40 kDa) in human tissue. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Host Mouse
Clonality Monoclonal
Clone BA-17
Isotype IgG1
Target Name Cytokeratin 19
Immunogen Mammary organoids.
Conjugation Un-conjugated
Alternate Names Cytokeratin-19; K19; CK-19; K1CS; Keratin-19; CK19; Keratin, type I cytoskeletal 19

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 5 µg/ml
ICC/IFAssay-dependent
IHC-Fr5 - 10 µg/ml
IHC-P5 - 10 µg/ml
IPAssay-dependent
WB1 - 2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3880 Human KRT19

Swiss-port # P08727 Human Keratin, type I cytoskeletal 19

Gene Symbol KRT19
Gene Full Name keratin 19, type I
Background Cytokeratin 19 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
Function Cytokeratin 19 involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Highlight Related products:
Cytokeratin 19 antibodies; Anti-Mouse IgG secondary antibodies;
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Research Area Signaling Transduction antibody
Calculated MW 44 kDa

Customer's Feedback

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Review for anti-Cytokeratin 19 antibody [BA-17]

Application:WB

Sample:MCF7 cell lysate

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Ocular melanoma and mammary mucinous carcinoma in an African lion.

IHC / Human

Cagnini DQ et al.
BMC Vet Res.,  (2012)

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Trp53 deletion stimulates the formation of metastatic pancreatic tumors.

IHC-P / Human

Morton JP et al.
Am J Pathol.,  (2008)

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Cytokeratin-18 is a useful serum biomarker for early determination of response of breast carcinomas to chemotherapy.

Olofsson MH et al.
Clin Cancer Res.,  (2007)

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Cellular immune reaction in the pancreas is induced by constitutively active IkappaB kinase-2.

IHC-Fr / Mouse

Aleksic T et al.
Gut.,  (2007)

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Oncostatin M, a cytokine released by activated mononuclear cells, induces epithelial cell-myofibroblast transdifferentiation via Jak/Stat pathway activation.

WB / Human

Nightingale J et al.
J Am Soc Nephrol.,  (2004)

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