ARG62462
anti-Cytokeratin 20 antibody [Ks20.8]
anti-Cytokeratin 20 antibody [Ks20.8] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [Ks20.8] recognizes Cytokeratin 20 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Specificity | This antibody is highly specific to cytokeratin 20 and shows no cross-reaction with other intermediate filament proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Ks20.8 |
| Isotype | IgG2a |
| Target Name | Cytokeratin 20 |
| Antigen Species | Human |
| Immunogen | Cytoskeletal preparation from micro-dissected villi of human duodenal mucosa. |
| Conjugation | Un-conjugated |
| Alternate Names | KRT21; CK20; K20; CD20; CK-20; Keratin-20; Cytokeratin-20; Keratin, type I cytoskeletal 20; Protein IT |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.3), Van Gogh Yellow Diluent and 0.1% Sodium azide |
| Preservative | 0.1% Sodium azide |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT20 |
| Gene Full Name | keratin 20, type I |
| Background | Cytokeratin 20 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine. [UniProt] |
| Cellular Localization | Cytoplasm |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 48 kDa |
| PTM | Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity). Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228. |
Clone References
Miliary brain metastases from occult lung adenocarcinoma: Radiologic and histopathologic confirmation.
IHC / Human
Three differentiation states risk-stratify bladder cancer into distinct subtypes.
IHC-Fr / Human
Huntingtin-interacting protein 1: a Merkel cell carcinoma marker that interacts with c-Kit.
IHC / Mouse
Expansion of CD133(+) colon cancer cultures retaining stem cell properties to enable cancer stem cell target discovery.
FACS / Human
Reconstitution of human telomerase reverse transcriptase expression rescues colorectal carcinoma cells from in vitro senescence: evidence against immortality as a constitutive trait of tumor cells.
ICC/IF / Human
Studying simple epithelial keratins in cells and tissues.
WB / Human
