ARG62980

anti-Cytokeratin 5 + 8 antibody [C-50]

anti-Cytokeratin 5 + 8 antibody [C-50] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Bovine,Dog,Hamster,Human,Mouse,Pig,Rat,Sheep

Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [C-50] recognizes Cytokeratin 5 + 8
Tested Reactivity Hu, Ms, Rat, Bov, Dog, Hm, Pig, Sheep
Species Does Not React With Chk, Xenopus laevis
Tested Application ICC/IF, IHC-P, IP, WB
Specificity The antibody C-50 reacts with cytokeratin peptides 5 and 8 (58 and 53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-50
Isotype IgG1
Target Name Cytokeratin 5 + 8
Immunogen Cytoskeleton preparation from HeLa cells.
Conjugation Un-conjugated
Alternate Names DDD1; 58 kDa cytokeratin; Keratin, type II cytoskeletal 5; EBS2; Keratin-5; Cytokeratin-5; CK5; KRT5A; K5; CK-5; Type-II keratin Kb5; DDD

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control IHC-P: Human prostate

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 110308 Mouse KRT5

GeneID: 281268 Bovine KRT5

GeneID: 369017 Rat KRT5

Gene Symbol KRT5
Gene Full Name keratin 5, type II
Background Cytokeratin 5 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
Research Area Signaling Transduction antibody
Calculated MW 62 kDa

Clone References

Human breast fibroblasts inhibit growth of the MCF10AT xenograft model of proliferative breast disease.

ICC/IF / Human

Sadlonova A et al.
Am J Pathol.,  (2007)

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Corticosteroids regulate epithelial cell differentiation and Hassall body formation in the human thymus.

IHC / Human

Hale LP et al.
J Immunol.,  (2004)

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Sulfur mustard induces the formation of keratin aggregates in human epidermal keratinocytes.

WB / Human

Dillman JF 3rd1 et al.
Toxicol Appl Pharmacol.,  (2003)

publication_link

 

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A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology.

Bártek J et al.
J Pathol.,  (1991)

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