ARG62981

anti-Cytokeratin 7 + 17 antibody [C-46]

anti-Cytokeratin 7 + 17 antibody [C-46] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Pig,Bovine

CK7/CK20 Carcinoma Study antibody

Overview

Product Description Mouse Monoclonal antibody [C-46] recognizes Cytokeratin 7 + 17
Tested Reactivity Hu, Bov, Pig
Species Does Not React With Ms, Rat, Rb
Tested Application ICC/IF, IHC-P, IP, WB
Specificity The clone C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues.
Host Mouse
Clonality Monoclonal
Clone C-46
Isotype IgG1
Target Name Cytokeratin 7 + 17
Antigen Species Human
Immunogen Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Conjugation Un-conjugated
Alternate Names SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
IHC-P10 µg/ml
IPAssay-dependent
WB1 - 2 µg/ml
Application Note WB: Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing condition).
IHC-P: Pretreatment: 0.1% pepsin for 30 min at 37°C. Incubation: 4°C overnight. Application note: Chromogen DAB.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: HeLa
IHC-P: Pancreas

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3855 Human KRT7

GeneID: 535697 Bovine KRT7

Swiss-port # P08729 Human Keratin, type II cytoskeletal 7

Swiss-port # Q29S21 Bovine Keratin, type II cytoskeletal 7

Gene Symbol KRT7
Gene Full Name keratin 7, type II
Background Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Function Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt]
Research Area CK7/CK20 Carcinoma Study antibody
Calculated MW 51 kDa
PTM Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

Clone References

Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis.

Sun Y et al.
Toxicology.,  (2009)

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A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.

Vojtĕsek B et al.
Neoplasma.,  (1990)

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