ARG62981
anti-Cytokeratin 7 + 17 antibody [C-46]
anti-Cytokeratin 7 + 17 antibody [C-46] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Pig,Bovine
CK7/CK20 Carcinoma Study antibody
Overview
| Product Description | Mouse Monoclonal antibody [C-46] recognizes Cytokeratin 7 + 17 |
|---|---|
| Tested Reactivity | Hu, Bov, Pig |
| Species Does Not React With | Ms, Rat, Rb |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Specificity | The clone C-46 reacts with Cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are a member of intermediate filaments subfamily represented in epithelial tissues. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | C-46 |
| Isotype | IgG1 |
| Target Name | Cytokeratin 7 + 17 |
| Antigen Species | Human |
| Immunogen | Cytoskeleton preparation of HeLa human cervix carcinoma cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Sample preparation: Lysis 1% lauryl maltoside for 1 hour, 5x10 sec. sonication; 5 min. in hot Laemmli buffer (reducing condition). IHC-P: Pretreatment: 0.1% pepsin for 30 min at 37°C. Incubation: 4°C overnight. Application note: Chromogen DAB. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | WB: HeLa IHC-P: Pancreas |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from hybridoma culture supernatant by protein A-affinity chromatography. |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS (pH 7.4) and 15 mM Sodium azide |
| Preservative | 15 mM Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KRT7 |
| Gene Full Name | keratin 7, type II |
| Background | Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| Function | Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt] |
| Research Area | CK7/CK20 Carcinoma Study antibody |
| Calculated MW | 51 kDa |
| PTM | Arg-20 is dimethylated, probably to asymmetric dimethylarginine. |
Clone References
Aberrant cytokeratin expression during arsenic-induced acquired malignant phenotype in human HaCaT keratinocytes consistent with epidermal carcinogenesis.
A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis.
