ARG62463

anti-Cytokeratin 7 antibody [OV-TL 12/30]

anti-Cytokeratin 7 antibody [OV-TL 12/30] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Human

CK7/CK20 Carcinoma Study antibody
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Overview

Product Description

Mouse Monoclonal antibody [OV-TL 12/30] recognizes Cytokeratin 7

Tested Reactivity Hu
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, WB
Specificity This antibody reacts with the 54 kD cytokeratin 7 intermediate filament protein
Host Mouse
Clonality Monoclonal
Clone OV-TL 12/30
Isotype IgG1, kappa
Target Name Cytokeratin 7
Antigen Species Human
Immunogen OTN 11, ovarian carcinoma cell line
Conjugation Un-conjugated
Alternate Names SCL; Sarcolectin; CK-7; Cytokeratin-7; CK7; Keratin, type II cytoskeletal 7; Keratin-7; K7; K2C7; Type-II keratin Kb7

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/10^6 cells
ICC/IF1:25 - 1:50
IHC-Fr1:25 - 1:50
IHC-P1:25 - 1:50
WB1:50 - 1:150
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 20 mM tris-borate (pH 7.5), 150 mM sodium chloride, dialyzed media RPMI 1640/D-MEM, fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein and 0.1% Sodium azide
Preservative 0.1% Sodium azide
Stabilizer fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3855 Human KRT7

Swiss-port # P08729 Human Keratin, type II cytoskeletal 7

Gene Symbol KRT7
Gene Full Name keratin 7, type II
Background Cytokeratin 7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
Function Cytokeratin 7 blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). [UniProt]
Cellular Localization Cytoplasm
Research Area CK7/CK20 Carcinoma Study antibody
Calculated MW 51 kDa
PTM Arg-20 is dimethylated, probably to asymmetric dimethylarginine.

Customer's Feedback

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Review for anti-Cytokeratin 7 antibody [OV-TL 12/30]

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:50

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Clone References

Serrated pathway adenocarcinomas: molecular and immunohistochemical insights into their recognition.

IHC / Human

Gurzu S et al.
PLoS One.,  (2013)

publication_link

 

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Mammalian target of rapamycin signalling modulates amino acid uptake by regulating transporter cell surface abundance in primary human trophoblast cells.

WB / Human

Rosario FJ et al.
J Physiol.,  (2013)

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Derivation and characterization of matched cell lines from primary and recurrent serous ovarian cancer.

WB / Human

Létourneau IJ et al.
BMC Cancer.,  (2012)

publication_link

 

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Immunohistochemical comparative study of fibrosis and biliary ductular reaction in alcoholic and viral chronic hepatitis.

IHC / Human

Egyed-Zsigmond I et al.
Rom J Morphol Embryol.,  (2010)

publication_link

 

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Fusion of villous trophoblast can be visualized by localizing active caspase 8.

ICC/IF / Human

Gauster M et al.
Placenta.,  (2009)

publication_link

 

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