ARG53215

anti-Cytokeratin 8 antibody

anti-Cytokeratin 8 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Signaling Transduction antibody
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Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 8
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 8
Antigen Species Human
Immunogen Recombinant protein corresponding to aa. 1-483 of Human Cytokeratin 8.
Conjugation Un-conjugated
Alternate Names Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB 1:25
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 10 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Breast Carcinoma, Lung, Skin, HeLa Cell Lysate

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Background Keratin 8 belongs to the type B (basic) subfamily of high molecular weight keratins and exists in combination with keratin 18. Keratin 8 is primarily found in the non-squamous epithelia and is present in majority of adenocarcinomas and ductal carcinomas. It is absent in squamous cell carcinomas. Hepatocellular carcinomas are defined by the use of antibodies that recognize only cytokeratin polypeptides 8 and 18.
Cellular Localization Cytoplasm, Membrane
Research Area Cancer antibody; Signaling Transduction antibody
Calculated MW 54 kDa
PTM Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.

Customer's Feedback

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Review for anti-Cytokeratin 8 antibody

Application:WB

Sample:HeLa

Sample Loading Amount:20 µg

Primary Antibody Dilution Factor:1:25

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC