ARG59499

anti-Cytokeratin 8 antibody

anti-Cytokeratin 8 antibody for Western blot,ICC/IF and Human,Mouse

Overview

Product Description Rabbit Polyclonal antibody recognizes Cytokeratin 8
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Cytokeratin 8
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-483 of Human Cytokeratin 8 (NP_002264.1).
Conjugation Un-conjugated
Alternate Names Keratin, type II cytoskeletal 8; KO; CYK8; CK-8; Type-II keratin Kb8; K2C8; CARD2; Keratin-8; K8; CK8; Cytokeratin-8

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa and NIH/3T3
Observed Size 53 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16691 Mouse KRT8

GeneID: 3856 Human KRT8

Swiss-port # P05787 Human Keratin, type II cytoskeletal 8

Swiss-port # P11679 Mouse Keratin, type II cytoskeletal 8

Gene Symbol KRT8
Gene Full Name keratin 8, type II
Background This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012]
Function Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. [UniProt]
Cellular Localization Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix. [UniProt]
Calculated MW 54 kDa
PTM Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.

O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.

O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner. [UniProt]